7, 10, 11 Tail transection of the larval zebrafish provides an accessible regeneration model with a variety of genetic variants to mimic immune deficiencies 12 mutations and morpholino knockdown methodologies permit the assessment of various cellular components in the wound signaling, healing and regrowth response, 7 while fluorescently labeled proteins allow for imaging cellular responses. The regenerative capacity and functional innate immune system of zebrafish larvae make them ideally suited for examining both the early events in the wound response and the later events of healing and regrowth.
![optixcam ocview detect camera optixcam ocview detect camera](https://tl360.b-cdn.net/wp-content/uploads/2021/11/Detect-Hidden-Spy-Cameras.jpg)
3, 7 Improved knowledge in this area could permit the development of treatments to support wound healing, especially in patients with suppressed immune systems and impaired wound healing responses. 3 Understanding the relationships between initial wound signals, the immune response, healing, and subsequent regrowth of damaged tissue has clinical relevance because a variety of disease states result in substantial tissue damage, 3 chronic inflammation 3, 6 and impaired wound healing. W ound healing is a dynamic process involving an orchestrated interaction of growth factors, 1 immune responses, 2, 3 extracellular matrix remodeling, 4 cell migration, 3 cell proliferation, 5 and differentiation. Finally, the zWEDGI was designed and fabricated using readily available techniques so that it can be easily modified for diverse experimental imaging protocols. Furthermore, we show that this device was amenable to long-term (>24 h) confocal microscopy of the caudal fin.
![optixcam ocview detect camera optixcam ocview detect camera](https://upload.ecvv.com/upload/Product/20118/China_Bathroom_spy_camera_kajoin_Motion_Detection_Hidden20118222150138.jpg)
The quality of tail fiber SHG images collected from larvae in the device was similar to fixed samples but provided the benefit of time lapse data collection. In this device, larval viability was maintained and tail regrowth was improved over embedding in agarose. To overcome this challenge, we engineered a functionally compartmentalized device, the zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI), to orient larvae for high-resolution microscopy, including confocal and second harmonic generation (SHG), while allowing unrestrained tail development and regrowth. The traditional strategy of larval encapsulation in agarose successfully limits sample movement but impedes larval development and tissue regrowth and is therefore not amenable to long-term imaging of wound healing. However, long-term imaging of wound healing poses technical challenges as wound healing occurs over multiple temporal scales.
![optixcam ocview detect camera optixcam ocview detect camera](https://static.hub.91mobiles.com/wp-content/uploads/2018/07/OPPO-Find-X-camera_thumb.jpg)
Zebrafish, an established model organism in developmental biology, is also a valuable tool for imaging wound healing in space and time with cellular resolution.